Plant tissue culture is a potent technology that enables researchers to work with plant cells and tissues under carefully controlled conditions. Its uses include the industrial manufacturing of valuable substances, the preservation of endangered species, genetic editing, and mass plant reproduction. This method has transformed plant science and has had a significant impact on biotechnology, horticulture, and agriculture. “Plant tissue culture : Relevance to Propagation, conservation and crop improvement” is a comprehensive book that explores the principles, techniques, and applications of plant tissue culture. It provides a detailed overview of the fundamental concepts underlying tissue culture, Molecular Markers, Stress tolerance and Mutation breeding. Furthermore, the book highlights the importance of plant tissue culture and Nanotechnology and tissue culture. This book will be equally useful to readers from a variety of backgrounds, including researchers, instructors, students, and biological scientists.