Human body is built of about one trillion cells. Anucleate cells of Acetabularia are capable of performing morphogenesis over a period of more than four weeks after removal of the nucleus. This book describes the methods for the isolation and characterization of anucleate mammalian cells and the techniques for the fusion of anucleate and nucleated cells; and outlines certain of the potential experimental applications of anucleate mammalian cell systems. The book will be very useful for students and beginners to gain insight into new area, as well as for experts and scientists to find new facts and expand their scientific horizons through biological sciences and biomedicine.