This book aims to give detailed information on various instruments, techniques and experiment protocols of microbiology and soil biology. The beauty of this book as it comprises chapters for the beginner’s viz. basic microbiological techniques and media preparation for biologists as well as the incorporation of advanced techniques for post graduate and research scholars. This laboratory manual gives a comprehensive idea about the various instruments, their working, troubleshooting and their applications based on student’s feedback, teacher’s input and authors own experiences of 14 years of teaching and research.